Tag Archives: Exposure

BEST PAPER AWARD at International Conference (PURE 2015, Malaysia)最優秀論文賞


11 Nov. 2015. BEST PAPER AWARD, 2nd International Conference on Purity, Utility Reaction and Environmental Research (PURE 2015), Kuala Lumpur.12265664_1658963404361849_7010463302459411147_o
“Development of a battery-driven passive sampling system for electrostatically trapping atmospheric particles”
(M. P. Khairunnisa, F. Faizal, H. Fukushima, M. Gen, P. Widiatmoko, I. W. Lenggoro*)



Full paper : Journal of Purity, Utility Reaction and Environment, 5(2), 43-50 (2016.Apr)

Open Access:


The related study was also honored as BEST POSTER AWARD during 12th Forum of Young Association of JAAST (Japan Association of Aerosol Science and Technology) 第12回エアロゾル若手フォーラム 2015/10/14 (Keio Univ.)

“A battery- and electrostatic-driven system for sampling suspended particles on a substrate”
(M. P. Khairunnisa, Ferry Faizal, Hisako Fukushima, Masao Gen*, Pramujo Widiatmoko**, I. Wuled Lenggoro)
Tokyo University of Agriculture and Technology; *Tohoku University; **Institut Teknologi Bandung.


Masao Gen: Doctoral presentation (5/23), 博士論文公聴会

Immobilization of submicron- and nano-particles from gas phase onto solid surfaces by external forces (外力を用いたサブミクロン粒子とナノ粒子の気相から固体基板への固定化)(Thesis is written in English)

2014/5/23 Friday, 15:00 ~ 16:30, BASE1階会議室

発表者: 玄 大雄 (GEN, Masao) **

** After working with WPI-AIMR, Tohoku University as Assistant Professor, he joined City University of Hong Kong

Keywords: Deposition/assembly of particles, Particle exposure system for plant growth chamber, Detector for residue pesticide


GEN, MASAO (玄 大雄)
Dr.Eng. 博士(工学)
Graduated in June 2014, Doctor of Engineering,
Grad. School of Bio-Applications and Systems Engineering (BASE).
東京農工大学 大学院 生物システム応用科学府 博士課程

  • Doctoral course (from 2011/10-2014/6), 博士課程後期(短縮修了)
    (Primary Supervisor: Wuled Lenggoro)
  • Master course (2010/4-2011/9), 博士課程前期(短縮修了)
    Thesis : External force-assisted assembly of aerosol particles on a substrate
    外力を用いたエアロゾル粒子の固体基板への集積 (in English)
    (Primary Supervisor: Wuled Lenggoro)
  • Bachelor course (Department of Chemical Engineering, TUAT, 2006/4-2010/3)
    東京農工大学 工学部 化学システム工学科 卒業
    (Primary Supervisor: Wuled Lenggoro)
  • Visiting doctoral student at Universiti Putra Malaysia (June-July 2012)
    (Host: Prof. M. Pauzi Zakaria, Faculty of Environmental Studies
    and Dr. M. Nazli bin Naim, Department of Process and Food Engineering)
    Sponsor: TUAT/FOLENS program

Iinoya Award 2013 for Masao Gen

2013: Iinoya Award (from JAAST)
Development of a portable sensor system based on aerosol particles to detect pesticide residues

2013: Kanji Takahashi Award from JAAST (As co-author),
Article published in Journal of Aerosol Research (Earozoru Kenkyu, 2011)
(Authors: Hama, Takashima, Gen, Tsukada, Kamiya, Lenggoro)

2013: Project leader of TUAT Innovation Organization Program Grant
“Development of a sensor system based to detect pesticide residues”
(文部科学省実践型研究リーダー養成事業 「ニーズ展開実践型高度研究人材養成モデル化事業」平成25年度プログラムの一環)

2012: TUAT FOLENS Program Travel Grant – for stay (30 days) Universiti Putra Malaysia. (FOLENS report)

2011: Certificate of Commendation from University (TUAT) president for work on “Design and fabrication of aerosol system for plants-growth chamber”. 農学府と連携した「粒子発生装置付き植物育成システム」に関する研究成果発表(APCChE-13)で学長からの表彰状。

2010: 13th Asia Pacific Confederation of Chemical Engineering (APCChE-13)
BEST POSTER PAPER AWARD (Category: Clean Energy Systems and The Environment)
“A plant-growth chamber system for the exposure to submicron aerosol particles”
(Lenggoro, Gen, Lim, Ikawa, Yamaguchi, Izuta)

* Society of Chemical Engineers, Japan
* Japan Association of Aerosol Science and Technology
* Pesticide Science Society of Japan

A colloidal route to detection of organic molecules based on surface-enhanced Raman spectroscopy using nanostructured substrate derived from aerosols,
M. Gen, H. Kakuta, Y. Kamimoto, I. W. Lenggoro,
Jpn. J. Appl. Phys. 50 (2011) 06GG10 (5 pages)

Simultaneous deposition of submicron aerosols onto both surfaces of a plate substrate by electrostatic forces
M. Gen, S. Ikawa, S. Sagawa, I. W. Lenggoro
e-Journal of Surface Science and Nanotechnology
Vol. 12 (2014) p. 238-241

Plant growth chamber system for submicron aerosol delivery to the leaf surfaces,
M. Gen*, S. Ikawa*, M. Yamaguchi, F. Lim, T. Izuta, I. W. Lenggoro,
*Equal contribution

Area-selective deposition of charged particles derived from colloidal aerosol droplets on a surface with different hydrophilic levels
K. Kusdianto, M. Gen, I. W. Lenggoro
Journal of Aerosol Science (2014)

Transformation of cyclodextrin glucanotransferase (CGTase) from aqueous suspension to fine solid particles via electrospray method
Enzyme and Microbial Technology (2014)
Saallah, Naim, Mokhtar, Abu Bakar, Gen, Lenggoro

Probing a dip-coated layer of organic molecules by aerosol nanoparticles sensor with sub-100 nm resolution based on surface-enhanced Raman scattering
M. Gen & I. W. Lenggoro (RSC Advances, 2014)

A protocol for detection of chemical residues distributed in a field using surface-enhanced Raman spectroscopy method,
M. Gen, H. Kakuta, Y. Kamimoto, I. W. Lenggoro (submitted)

Surface-enhanced Raman spectroscopy from metal nanoparticles derived from charged aerosols,
M. Gen and I. W. Lenggoro (submitted)

Vacuum-induced rearrangement of pattern of metal nanoparticles deposited on a flat substrate
M. Gen and I. W. Lenggoro (Submitted)

Micro-Raman investigation on the hygroscopic behavior in nanoparticles filled ammonium sulfate particle deposited on a substrate
I. W. Lenggoro, E. Herliana, M. Gen, A. Seki (submitted)

Generation of nano and submicro-meter sized carbonaceous aerosol particles
S. Ikawa*, M. Gen*, I. W. Lenggoro (submitted) *: Equal contribution

Effects of structures of dilution samplers and operating conditions on aerosol formation from CdCl2 vapor gas
H. Kamiya, M. Gen, T. Sekizawa, K. Hada, T. Ejima, M. Tsukada, I. W. Lenggoro (submitted)

Measurement of aerosols derived from model gas containing two metal components after cooling and dilution process
N. Hama, Y. Takashima, M. Gen, M. Tsukada, H. Kamiya, I. W. Lenggoro
Earozoru Kenkyu (Journal of Aerosol Research Japan), 26(4) 277-285 (2011)

IAC (International Aerosol Conference) Busan, Korea.
2014, August 28 to September 2
(Gen and Lenggoro)

IAC (International Aerosol Conference) Busan, Korea.
2014, August 28 to September 2
(Kus, Gen, Lenggoro)

8th Asian Aerosol Conference (2-5 December 2013) Sydney.
Aerosol-Assisted Characterization of Organic Molecules Adsorbed on a Solid Substrate
(Gen & Lenggoro)

5th Nanoscience and Nanotechnology Symposium, NNS,
23-25 October 2013 (Hotel Meritus, Surabaya, Indonesia)
CS-68. Fabrication of nanostructure derived from gas-phase nanoparticles for detection of organic molecules

5th Asian Particle Technology Symposium, Singapore 3-5 July 2012
172: Oral: Assembly of Gas-Phase Nanoparticles on a Structural Substrate for Spectroscopic-Based Sensor to Detect a Pesticide
Masao Gen*, Hideo Kakuta, Yoshihito Kamimoto and I. Wuled Lenggoro

Deposition of submicron aerosols on both sides of a substrate derived from charged droplets of aqueous suspension:
M. Gen, S. Sagawa, H. Kamiya, W. Lenggoro,
7th Asian Aerosol Conference, 2011/8/17-20, Xi’an (China)

Nanostructured substrates derived from aerosol nanoparticles for a tagging system with surface-enhanced Raman spectroscopy (Poster, 11D-8-59),
M. Gen, H. Kakuta, Y. Kamimoto, W. Lenggoro
23rd International Microprocesses and Nanotechnology Conference, 2010/11/9-12, Fukuoka

第78回化学工学会年会 2013/03/17

日本農薬学会第38回大会 2013/03/14

希釈器内における混合ガス由来のモデルエアロゾルの計測 (Invited)
COMSOL Conference Tokyo 2012, 2012/11/22

空気中に浮遊する微粒子の検出技術 ~ナノスケール構造体の形成から農薬散布のモニタリングまで~
東京農工大学 科学技術展 2012, 2012/11/09

微粒子汚染と植物への影響 -モデル微粒子の生成と沈着が可能な装置の開発-
東京農工大工 科学技術展 2012, 2012/11/09

(東農工大院BASE)玄 大雄・ (植物情報物質研究セ) 角田 英男・ (神奈川県産技セ) 上元 好仁・ (東農工大院工) Wuled Lenggoro
化学工学会 第77年会, 2012/3/15

Front- and rear-side deposition of submicron aerosols derived from charged droplets containing particles (Poster)
Gen Masao, Sagawa Shin-ichi, Kamiya Hidehiro, Wuled Lenggoro
化学工学会 第43回秋季大会: 2011/9/14-16, Nagoya

玄 大雄・角田 英男・上元 好仁・Wuled Lenggoro
化学工学会 第43回秋季大会, 2011/9/14-16: Nagoya

W. Lenggoro, 玄 大雄, 佐川 慎一, 伊豆田 猛,
化学工学会秋季大会, 2010/9/6, Kyoto.

Electrical-assisted deposition of aerosol particles derived from aqueous suspension of nanopowders (3I05),
M. Gen, S. Sagawa, N. Naim, M. Iijima, H. Kamiya, W. Lenggoro*
セラミックス協会2010年会, 2010/3/24, Tokyo

玄 大雄, Lim Fong Zyin, 井川 誠司, 山口 真弘, 伊豆田 猛, Lenggoro Wuled*
第12回 化学工学会 学生発表会(東日本地区) 2010/3/6, Tokyo

OTHER PRESENTATIONS (as a co-author)
環境大気中PM2.5 の表面電位の測定
第30回エアロゾル科学・技術研究討論会 2013/08/27

第22回環境化学討論会 2013/07/31

Hygroscopic Behavior of Inorganic Particles Deposited on Solid Surfaces
9th Asia Pacific Conference on Sustainable Energy & Environmental Technologies (APCSEET 2013) 2013/07/05

A protocol to detect chemical residues using a nanoparticle-based sensor combined with a Raman spectroscopic method
19th Regional Symposium on Chemical Engineering, 2012/11/07

Hygroscopic behavior of inorganic particles deposited on solid surfaces
第29回エアロゾル科学・技術研究討論会, 2012/08/28

5th Asian Particle Technology Symposium, Singapore 3-5 July 2012
122: Oral: A Chamber System with Aerosol Generators for Exposures of Plants to Submicrometer-Sized Particles
Wuled Lenggoro*, Masao Gen, Seiji Ikawa, Fong-Zyin Lim, Masahiro Yamaguchi and Takeshi Izuta

An aerosol generator system for long-duration exposures of plants to submicrometer-sized particles
W. Lenggoro; M. Gen; F.-Z. Lim; S. Ikawa; M. Yamaguchi; T. Izuta
2011/8/17-20, Xi’an

A plant growth chamber system for the exposure to submicron aerosol particles
Wuled Lenggoro, Masao Gen, Fong-Zyin Lim, Seiji Ikawa, Masahiro Yamaguchi, Takeshi Izuta
13th Asia Pacific Confederation of Chemical Engineering Congress, 2010/10/6-8, Taipei

Lim Fong Zyin, 玄 大雄, 神谷 秀博, 伊豆田 猛, Wuled Lenggoro,
化学工学会秋季大会, 2010/9/7, Kyoto.

Wuled Lenggoro, 玄 大雄 , Lim Fong Zyin, 井川 誠司, 山口 真弘, 伊豆田 猛
2010エアロゾル科学技術研究討論会, 2010/8/4, Nagoya

佐川 慎一、玄 大雄、Nazli Naim、飯島志行、神谷秀博、Wuled Lenggoro,
2009エアロゾル科学技術研究討論会, 2009/8, Okayama

Other doctoral thesis from our group (Supervisor: Wuled Lenggoro at TUAT, since 2007)


|| Present member || Former member ||

PAPER: Effect of water-soluble particles on the growth of plants (2 years)…植物に水溶性微粒子を与えていたら?

JOURNAL: Atmospheric Environment (2014)

Effects of 2-years exposure to (sub-micron) ammonium sulfate particles on growth and gas exchange rates of Fagus crenata (ブナ), Castanopsis sieboldii (スダジイ), Larix kaempferi (唐松) and Cryptomeria japonica (スギ) seedlings

•No significant effect of the particles on growth was observed after 2 years.
•Net photosynthetic rates of 3 species were not significantly changed.
•Net photosynthesis of Cryptomeria japonica was relatively sensitive to (NH4)2SO4.
•Both increase and reduction in net photosynthetic rate were observed in C. japonica.


Four Japanese forest tree species were exposed to submicrometer (NH4)2SO4 particles.

A work from:
TUAT, Agriculture; TUAT, Engineering; Kyoto Univ.; Forestry and Forest Products Research Institute; Hokkaido Univ.

OTHER Recent Papers


Student as a project leader in a MEXT/TUAT program, 学生が研究代表者

Masao Gen, a doctoral student got a grant for his project on advancing a sensor technology to detect pesticide on-site. Sponsor = Innovation Advancement Organization (IAO), based on a TUAT/MEXT project.
The program includes few-days training at SRI (Stanford Research Institute, California) and Cornell University.



A reference of our Pesticide Detector (Gen et al., 2011)

PAPER. Measuring carbon particles on leaf surface, 葉面上の炭素系粒子の計測手法

Journal: Asian Journal of Atmospheric Environment (2012)

Optical method for measuring deposition amount of black carbon particles on foliar surface

M. Yamaguchi, K. Takeda, Y. Otani, N. Murao, H. Sase, I. W. Lenggoro, K. Yazaki, K. Noguchi, A. Ishida, T. Izuta

Full article (PDF):

研究機関: 東京農工大学(農), 北海道大学(工), 日本環境衛生センター/アジア大気汚染研究センター, 森林総合研究所, 東京農工大学(工), 京都大学生態学研究センター

1: Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo
2: Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo
3: Graduate School of Engineering, Hokkaido University, Sapporo, Hokkaido
4: Asia Center for Air Pollution Research, Niigata
5: Institute of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo
6: Forestry and Forest Products Research Institute, Tsukuba, Ibaraki
7: Shikoku Research Center, Forestry and Forest Products Research Institute, Kochi
8: Center for Ecological Research, Kyoto University, Otsu, Shiga
9: Institute of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo

To perform quick measurements of black carbon (BC) particles deposited on foliar surfaces of forest tree species, we investigated an optical method for measuring the amount of BC extracted from foliar surfaces and collected on quartz fiber filters. The seedlings of Fagus crenata, Castanopsis sieboldii, Larix kaempferi and Cryptomeria japonica were exposed to submicron BC particles for one growing season (June to December). At the end of the growing season, the leaves or needles of the seedlings were harvested and washed with deionized water followed by washing with chloroform to extract the BC particles deposited on the foliar surfaces. The extracted BC particles were collected on a quartz fiber filter. The absorption spectrum of the filters was measured by spectrophotometer with an integrating sphere. To obtain the relationship between the absorbance of the filter and the amount of BC particles on the filter, the amount of BC particles on the filter was determined as that of elemental carbon (EC) measured by a thermal optical method. At wavelengths below 450 nm, the absorption spectrum of the filter showed absorption by biological substances, such as epicuticular wax, resulting in the low coefficient of determination (R2) in the relationship between the amount of EC on the filter (MEC, μg C cm−2 filter area) and the absorbance of the filter. The intercept of the regression line between MEC and the absorbance of the filter at 580 nm (A580) was closest to 0. There was a significant linear relationship between the A580 and MEC (R2 = 0.917)

files-folderRecent topics from published journal articles: 最近の論文概要

Co-hosted: International Symposium on Aerosols in East Asia & Their Impacts on Plants & Human Health (11/29-12/1)

We co-hosted and most of students assisted an International Symposium held in our campus:

Aerosols in East Asia and Their Impacts on Plants and Human Health
Nov.29-Dec.1, TUAT Koganei campus


>> Symposium website

Presentations from our group:

0) A Plant Growth Chamber System Facilitated with Aerosol Depositor for Transporting Submicron Articles onto Leaf Surfaces.
M. Gen (D2), S. Ikawa (D3), F. Y. Lim, M. Yamaguchi, T. Izuta, W. Lenggoro

1) Collaborated with Izuta Group/Institute of Agriculture, etc.
1.1) Experimental study on the effects of ammonium sulfate particles on Fagus crenata, Castanopsis sieboldii, Larix kaempferi and Cryptomeria japonica
M. Yamaguchi, Y. Otani, P. Li, H. Nagao, A. Seki, I. W. Lenggoro, K. Yazaki, K. Noguchi, A. Ishida, T. Izuta

1.2) Effects of black carbon particles on growth and gas exchange rates of Fagus crenata, Castanopsis sieboldii, Larix kaempferi and Cryptomeria japonica seedlings
M. Yamaguchi, K. Takeda, Y. Otani, I. Wuled Lenggoro, K. Yazaki, K. Noguchi, A. Ishida, H. Sase, N. Murao, T. Izuta

2) Collaborated with Kamiya group, etc.
2.1.) Aerosol formation and emission behavior characterization from stationary sources
H. Kamiya, M. Tsukada, W. Lenggoro, N. Namiki, M. Wada, N. Noda, H. Makino, C. Minejima, W. W. Szymanski
1 Tokyo University of Agriculture and Technology, 2 Kogakuin University, 3 Ishikawa National College of Technology, 4 Central Research Institute of Electric Power Industry, 5 University of Vienna

2.2) Evaluation of fine particle emission from stationary sources by surface area concentration
M. Arai, M. Tsukada, M. Yamada, T. Ejima, T. Sekizawa, S. Ikawa, N. Hama, W. Lenggoro, H. Kamiya

Present at Jpn. Soc. Atmospheric Environment (13 Sep.), 植物成長 vs. 酸性粒子

PRESENTATION: Research with collaborators.
53rd Annual Conference of Japan Society for Atmospheric Environment

Experimental study on the influences of ammonium sulfate aerosol particles on four types of plants (in Japanese)

第 53 回大気環境学会年会 (2012年9月12-14日横浜)

・山口 真弘・大谷 葉子・竹田 健太・李 ホ ツゼン・長尾 宙・(M2)関 明人・半 智史・山根 健一(東京農工大学)
・矢崎 健一・野口 享太郎・黒田 克史(森林総合研究所)
・佐野 雄三(北海道大学)
・Wuled Lenggoro(東京農工大学)
・石田 厚(京都大学)
・船田 良・伊豆田 猛(東京農工大学)

Contribution from our group, by Seki (M2) and Lenggoro

Recent topics of published journal articles,最近の発表論文の概要…